Thalassaemia Lab Report Example | Topics and Well Written Essays - 500 words

thalassemia - Lab Report ExampleThen there is the -thalassaemia singularity characterized by two affected -globin genes. Hemoglobin H disease is the third type classification of -thalassaemia which has ternion affected genes. Finally, there is the -Thalassaemia Major that has four affected -globin gene (Weatherall and Clegg 2008).There be terce different types of -thalassaemia. The first matchless is -thalassemia trait which has one mutated -globin gene and one normal one. The second one is Thalassaemia Intermedia, which has two abnormal l genes although there is a little production of -globin. The third is Thalassaemia Major in which there are two abnormal genes that cause extreme decrease or complete lack of -globin production (Weatherall and Clegg 2008).Haemoglobin electrophoresis is a method used to breach the different blood haemoglobin types in a procedure that uses cellulose acetate in alkaline buffer store. The turn involves the use of extremely small samples of haem osylates prepared from whole samples of blood. These samples are applied to the Titan III Cellulose acetate rayon Plate whereby the haemoglobins in the samples undergo separation by electrophoresis with the use of an alkaline buffer. The pH of the buffer ranges between 8.2 and 8.6. They are then stained with Ponceau S Stain and the resulting patterns that form are scanned on a scanning densitometer followed by determining the relative percentage of each band.TF is diluted to 15ml, and SF is collected in 3ml so as to maximize absorption in the spectrometer. This, however, does not affect the results because they were diluted using distilled body of water. Distilled water is free of ions and other soluble contaminants and therefore, cannot affect the result of the experiment.The pH of the solvent in which amino acids are dissolved mostly determine their ionic state. Cationic amino acids exist at low solution pH of approximately 1. An increase in solution pH causes the